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cho k1 cell lines  (ATCC)


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    Structured Review

    ATCC cho k1 cell lines
    Cho K1 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cho+cell+lines/CHO-K1/pm42127664-574-19-23
    Average 99 stars, based on 7332 article reviews
    cho k1 cell lines - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Alternative Splicing:

    Article Title: Subphysiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells.
    Article Snippet: .. To determine if alternative splicing events could be identified in additional CHO cell lines, a non-producing CHO K1 cell line acquired from ATCC (CHO K1-ATCC) along with a second CHO K1 cell line producing an anti-IL8 mAb (CHO K1-DP12) were also analysed by qPCR. .. The CHO K1ATCC and CHO K1-DP12 cell lines were cultured using the same model of temperature shift previously described however these cells were maintained in BalanCD CHO Growth A Medium (Irvine Scientific, 91128) supplemented with 6mM and 8mM L-glutamine respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: Subphysiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells.
    Article Snippet: .. To determine if alternative splicing events could be identified in additional CHO cell lines, a non-producing CHO K1 cell line acquired from ATCC (CHO K1-ATCC) along with a second CHO K1 cell line producing an anti-IL8 mAb (CHO K1-DP12) were also analysed by qPCR. .. The CHO K1ATCC and CHO K1-DP12 cell lines were cultured using the same model of temperature shift previously described however these cells were maintained in BalanCD CHO Growth A Medium (Irvine Scientific, 91128) supplemented with 6mM and 8mM L-glutamine respectively.

    Ubiquitin Proteomics:

    Article Title: Predominant right temporal lobe atrophy: Clinical, neuropsychological and structural differences based on amyloid status.
    Article Snippet: .. A recent study by Mathias et al. (2020) demonstrated a role for the ubiquitin proteasome system via ER associated degradation in the degradation of difficult to express antibodies in CHO cell lines (Mathias et al., 2020).... Show abstract Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture , Journal of Bioscience and Bioengineering Citation Excerpt : ...CHO DP-12 clone no. 1934 (ATCC CRL-12445) cells producing the anti-IL8 antibody (18) were used.... Show abstract Reinventing the Wheel: Synthetic Circular RNAs for Mammalian Cell Engineering , Trends in Biotechnology Citation Excerpt : ...Of yet there has been no report to our knowledge of engineering production cell systems by exogenous expression or repression of naturally occurring circular RNAs. ..

    Polymer:

    Article Title: Predominant right temporal lobe atrophy: Clinical, neuropsychological and structural differences based on amyloid status.
    Article Snippet: .. A recent study by Mathias et al. (2020) demonstrated a role for the ubiquitin proteasome system via ER associated degradation in the degradation of difficult to express antibodies in CHO cell lines (Mathias et al., 2020).... Show abstract Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture , Journal of Bioscience and Bioengineering Citation Excerpt : ...CHO DP-12 clone no. 1934 (ATCC CRL-12445) cells producing the anti-IL8 antibody (18) were used.... Show abstract Reinventing the Wheel: Synthetic Circular RNAs for Mammalian Cell Engineering , Trends in Biotechnology Citation Excerpt : ...Of yet there has been no report to our knowledge of engineering production cell systems by exogenous expression or repression of naturally occurring circular RNAs. ..

    Derivative Assay:

    Article Title: Predominant right temporal lobe atrophy: Clinical, neuropsychological and structural differences based on amyloid status.
    Article Snippet: .. A recent study by Mathias et al. (2020) demonstrated a role for the ubiquitin proteasome system via ER associated degradation in the degradation of difficult to express antibodies in CHO cell lines (Mathias et al., 2020).... Show abstract Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture , Journal of Bioscience and Bioengineering Citation Excerpt : ...CHO DP-12 clone no. 1934 (ATCC CRL-12445) cells producing the anti-IL8 antibody (18) were used.... Show abstract Reinventing the Wheel: Synthetic Circular RNAs for Mammalian Cell Engineering , Trends in Biotechnology Citation Excerpt : ...Of yet there has been no report to our knowledge of engineering production cell systems by exogenous expression or repression of naturally occurring circular RNAs. ..

    Article Title: Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum-free media for Chinese hamster ovary cells.
    Article Snippet: While reliable transfection methods are essential for Chinese Hamster Ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed in several commercially-prepared media.. In this study, we aimed to assess common media additives that impede efficiency mediated by three chemical transfection agents: liposomal-based (Lipofectamine 2000), polymer-based (TransIT-X2), and lipopolyplex-based (TransIT-PRO).. An in-house GFP-expressing vector and serum-free medium (BCR-F12: developed for the purposes of this study), were used to analyze transient transfection efficiencies of three CHO cell lines (CHO-K1, DG44, DP12).

    Expressing:

    Article Title: Predominant right temporal lobe atrophy: Clinical, neuropsychological and structural differences based on amyloid status.
    Article Snippet: .. A recent study by Mathias et al. (2020) demonstrated a role for the ubiquitin proteasome system via ER associated degradation in the degradation of difficult to express antibodies in CHO cell lines (Mathias et al., 2020).... Show abstract Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture , Journal of Bioscience and Bioengineering Citation Excerpt : ...CHO DP-12 clone no. 1934 (ATCC CRL-12445) cells producing the anti-IL8 antibody (18) were used.... Show abstract Reinventing the Wheel: Synthetic Circular RNAs for Mammalian Cell Engineering , Trends in Biotechnology Citation Excerpt : ...Of yet there has been no report to our knowledge of engineering production cell systems by exogenous expression or repression of naturally occurring circular RNAs. ..

    Article Title: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates.
    Article Snippet: .. Generation of stable CHO cell lines expressing two BG505 UFO-Env NP vaccine candidates Stable CHO cell lines expressing BG505 UFO-E2p-L4P and UFO-10GSI3-01v9-L7P NPs were generated by the cationic lipid mediated transfection of a parental suspension culture adopted CHO-K1 host cell line (ATCC no. CCL-61) using bacterial artificial chromosomes (BACs) as gene transfer vehicles157. ..

    Neutralization:

    Article Title: Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells
    Article Snippet: .. Furthermore, C1s −/− and MGAT1 −/− double-knocked CHO cell lines (ATCC:CCL-61) can efficiently produce untailored gp120 and enrich multiple mannose-5 glycans necessary for widespread neutralization of mAb binding (Li et al. , ). ..

    Binding Assay:

    Article Title: Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells
    Article Snippet: .. Furthermore, C1s −/− and MGAT1 −/− double-knocked CHO cell lines (ATCC:CCL-61) can efficiently produce untailored gp120 and enrich multiple mannose-5 glycans necessary for widespread neutralization of mAb binding (Li et al. , ). ..

    Recombinant:

    Article Title: Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum-free media for Chinese hamster ovary cells.
    Article Snippet: While reliable transfection methods are essential for Chinese Hamster Ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed in several commercially-prepared media.. In this study, we aimed to assess common media additives that impede efficiency mediated by three chemical transfection agents: liposomal-based (Lipofectamine 2000), polymer-based (TransIT-X2), and lipopolyplex-based (TransIT-PRO).. An in-house GFP-expressing vector and serum-free medium (BCR-F12: developed for the purposes of this study), were used to analyze transient transfection efficiencies of three CHO cell lines (CHO-K1, DG44, DP12).

    Suspension:

    Article Title: Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum-free media for Chinese hamster ovary cells.
    Article Snippet: While reliable transfection methods are essential for Chinese Hamster Ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed in several commercially-prepared media.. In this study, we aimed to assess common media additives that impede efficiency mediated by three chemical transfection agents: liposomal-based (Lipofectamine 2000), polymer-based (TransIT-X2), and lipopolyplex-based (TransIT-PRO).. An in-house GFP-expressing vector and serum-free medium (BCR-F12: developed for the purposes of this study), were used to analyze transient transfection efficiencies of three CHO cell lines (CHO-K1, DG44, DP12).

    Article Title: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates.
    Article Snippet: .. Generation of stable CHO cell lines expressing two BG505 UFO-Env NP vaccine candidates Stable CHO cell lines expressing BG505 UFO-E2p-L4P and UFO-10GSI3-01v9-L7P NPs were generated by the cationic lipid mediated transfection of a parental suspension culture adopted CHO-K1 host cell line (ATCC no. CCL-61) using bacterial artificial chromosomes (BACs) as gene transfer vehicles157. ..

    Cell Culture:

    Article Title: Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum-free media for Chinese hamster ovary cells.
    Article Snippet: While reliable transfection methods are essential for Chinese Hamster Ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed in several commercially-prepared media.. In this study, we aimed to assess common media additives that impede efficiency mediated by three chemical transfection agents: liposomal-based (Lipofectamine 2000), polymer-based (TransIT-X2), and lipopolyplex-based (TransIT-PRO).. An in-house GFP-expressing vector and serum-free medium (BCR-F12: developed for the purposes of this study), were used to analyze transient transfection efficiencies of three CHO cell lines (CHO-K1, DG44, DP12).

    Generated:

    Article Title: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates.
    Article Snippet: .. Generation of stable CHO cell lines expressing two BG505 UFO-Env NP vaccine candidates Stable CHO cell lines expressing BG505 UFO-E2p-L4P and UFO-10GSI3-01v9-L7P NPs were generated by the cationic lipid mediated transfection of a parental suspension culture adopted CHO-K1 host cell line (ATCC no. CCL-61) using bacterial artificial chromosomes (BACs) as gene transfer vehicles157. ..

    Transfection:

    Article Title: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates.
    Article Snippet: .. Generation of stable CHO cell lines expressing two BG505 UFO-Env NP vaccine candidates Stable CHO cell lines expressing BG505 UFO-E2p-L4P and UFO-10GSI3-01v9-L7P NPs were generated by the cationic lipid mediated transfection of a parental suspension culture adopted CHO-K1 host cell line (ATCC no. CCL-61) using bacterial artificial chromosomes (BACs) as gene transfer vehicles157. ..



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